Validated Methods

Validated Bioanalytical Assays

We have a wide range of clinical and preclinical non-proprietary methods which are validated to current regulatory guidelines. If a compound of interest is not listed, please contact us as we have vast experience in the development and validation of small and large molecules and we will usually be able to support your bioanalytical project at short notice.

Our range of state-of-the-art instrumentation and technologies includes:

Waters Xevo™ TQ-XS and TQ-S LC-MS/MS systems
AB SCIEX API 4000™, 5000™ and TQ 6500+™ LC-MS/MS systems
Waters Acquity I-Class UPLC™ and Shimadzu Nexera UHPLC systems
Hamilton MicroLab Star robotics systems
Spark-Holland Symbiosis™ Pharma systems
Immunoassay
MSD systems
Watson LIMS and Labnotes ELN

Validated Bioanalytical Methods

Compound	LLOQ	ULOQ	Species & Matrix	Type
1-Hydroxymidazolam	0.1 ng/mL	100 ng/mL	Human Plasma	LC-MS/MS
4'-Hydroxyflurbiprofen	0.25 µg/mL	25 µg/mL	Human Urine	LC-MS/MS
4-Hydroxytolbutamide	10 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
5-Aminosalicylic Acid (ASA)	5.0 µg/mL	1,000 µg/mL	Human Urine	LC-MS/MS
5-Aminosalicylic Acid (ASA)	5.0 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
5-Hydroxyomeprazole	5.0 ng/mL	2,000 ng/mL	Human Plasma	LC-MS/MS
6ß-Hydroxycortisol	200 ng/mL	35,000 ng/mL	Human Urine	LC-MS/MS
7-Hydroxymethotrexate	5.0 ng/mL	1,000 ng/mL	Human Plasma	LC-MS/MS
7-Hydroxymethotrexate	20 ng/mL	15,000 ng/mL	Human Urine	LC-MS/MS
9-Hydroxyrisperidone	0.1 ng/mL	100 ng/mL	Human Plasma	LC-MS/MS
17-Hydroxyexemestane	50 pg/mL	5,000 pg/mL	Human Plasma	LC-MS/MS
24,25-Dihydroxycholecalciferol	50 pg/mL	10,000 pg/mL	Human Plasma	LC-MS/MS
Adefovir	0.5 ng/mL	500 ng/mL	Human Plasma	LC-MS/MS
Amoxicillin	100 ng/mL	20,000 ng/mL	Human Plasma	LC-MS/MS
Atomoxetine	5 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
Budesonide	5.0 pg/mL	1,000 pg/mL	Human Serum	LC-MS/MS
Buprenorphine	1.0 pg/mL	1, 000 pg/mL	Human Plasma	LC-MS/MS
Caffeine	20 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
Calcifediol	1.0 ng/mL	200 ng/mL	Human Plasma	LC-MS/MS
Calcitriol	10 pg/mL	2,000 pg/mL	Human Plasma	LC-MS/MS
Candesartan	0.5 ng/mL	250 ng/mL	Human Plasma	LC-MS/MS
Ciprofloxacin	5.0 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
Clopidogrel carboxylic acid	5.0 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
Cortisol	5.0 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
Cortisol	40 ng/mL	7,000 ng/mL	Human Urine	LC-MS/MS
C-Reactive Protein (CRP)	1.0 ng/mL	100 ng/mL	Human Plasma	ELISA
Cyclosporin	20 ng/mL	4,000 ng/mL	Human Whole Blood	LC-MS/MS
Dextromethorphan	10 ng/mL	2,000 ng/mL	Human Urine	LC-MS/MS
Dextrorphan	100 ng/mL	20,000 ng/mL	Human Urine	LC-MS/MS
Digoxin	0.1 ng/mL	5.0 ng/mL	Human Plasma	LC-MS/MS
Dihydrotestosterone	100 pg/mL	5,000 ng/mL	Human Serum	LC-MS/MS
Diltiazem	1.0 ng/mL	250 ng/mL	Human Plasma	LC-MS/MS
Docetaxel	0.5 ng/mL	1,000 ng/mL	Human Plasma	LC-MS/MS
Exemestane	0.1 ng/mL	25 ng/mL	Human Plasma	LC-MS/MS
Flurbiprofen	0.1 µg/mL	20 µg/mL	Human Plasma	LC-MS/MS
Flurbiprofen	0.25 µg/mL	25 µg/mL	Human Urine	LC-MS/MS
Fluticasone Propionate	3.0 pg/mL	200 pg/mL	Human Plasma	LC-MS/MS
Fluticasone Propionate and Salmeterol	0.5 pg/mL	200 pg/mL	Human Plasma (200 µL)	LC-MS/MS
Glibenclamide	2.0 ng/mL	1,000 ng/mL	Human Plasma	LC-MS/MS
Glipizide	5.0 ng/mL	2,000 ng/mL	Human Plasma	LC-MS/MS
Hydroxyitraconazole	1.0 ng/mL	1,000 ng/mL	Human Plasma	LC-MS/MS
Hydroxymetronidazole	100 ng/mL	10,000 ng/mL	Human Plasma	LC-MS/MS
Itraconazole	1.0 ng/mL	1,000 ng/mL	Human Plasma	LC-MS/MS
Ketoconazole	10 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
Lamivudine	2.0 ng/mL	2,000 ng/mL	Human Plasma	LC-MS/MS
Lorazepam	1.0 ng/mL	150 ng/mL	Human Plasma	LC-MS/MS
Metformin	10 ng/mL	10,000 ng/mL	Human Plasma	LC-MS/MS
Methotrexate	5.0 ng/mL	1,000 ng/mL	Human Plasma	LC-MS/MS
Methotrexate	50 ng/mL	1,000 ng/mL	Human Urine	LC-MS/MS
Metronidazole	500 ng/mL	30,000 ng/mL	Human Plasma	LC-MS/MS
Midazolam	0.1 ng/mL	100 ng/mL	Human Plasma	LC-MS/MS
Moxifloxacin	50 ng/mL	7,500 ng/mL	Human Plasma	LC-MS/MS
N-Acetyl-5-Aminosalicylic Acid (NASA)	5.0 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
N-Acetyl-5-Aminosalicylic Acid (NASA)	5.0 µg/mL	5,000 µg/mL	Human Urine	LC-MS/MS
Norbuprenorphine	5.0 pg/mL	1,000 pg/mL	Human Plasma	LC-MS/MS
O-Desmethyl Venlafaxine	1.0 ng/mL	400 ng/mL	Human Plasma	LC-MS/MS
Omeprazole	5.0 ng/mL	2,000 ng/mL	Human Plasma	LC-MS/MS
Omeprazole Sulphone	5.0 ng/mL	2,000 ng/mL	Human Plasma	LC-MS/MS
Paraxanthine	20 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
Pioglitazone	25 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
Pioglitazone MIV	25 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
Pravastatin	0.1 ng/mL	100 ng/mL	Human Plasma	LC-MS/MS
Prednisolone	0.5 ng/mL	500 ng/mL	Human Plasma	LC-MS/MS
Prednisolone	20 ng/mL	20,000 ng/mL	Human Plasma	LC-MS/MS
Risperidone	0.1 ng/mL	100 ng/mL	Human Plasma	LC-MS/MS
Salbutamol	50 pg/mL	10,000 pg/mL	Human Plasma	LC-MS/MS
Salbutamol	3.0 ng/mL	1,000 ng/mL	Human Urine	LC-MS/MS
Salmeterol	5.0 pg/mL	1,000 pg/mL	Human Plasma	LC-MS/MS
Salmeterol	1.0 pg/mL	200 pg/mL	Human Plasma	LC-MS/MS
Salmeterol and Fluticasone Propionate	0.5 pg/mL	200 pg/mL	Human Plasma (200 µL)	LC-MS/MS
Simvastatin	0.05 ng/mL	100 ng/mL	Human Plasma	LC-MS/MS
Simvastatin Acid	0.05 ng/mL	100 ng/mL	Human Plasma	LC-MS/MS
Sitagliptin	5.0 ng/mL	5000 ng/mL	Human Plasma	LC-MS/MS
Sulfamethoxazole	1.0 µg/mL	100 µg/mL	Human Plasma	LC-MS/MS
Testosterone	250 pg/mL	50,000 pg/mL	Human Serum	LC-MS/MS
Testosterone	50 pg/mL	25,000 pg/mL	Human Serum	LC-MS/MS
Tolbutamide	10 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS
Venlafaxine	1.0 ng/mL	400 ng/mL	Human Plasma	LC-MS/MS
Warfarin (R & S)	20 ng/mL	5,000 ng/mL	Human Plasma	LC-MS/MS

Validated Methods

Validated Bioanalytical Assays

Validated Bioanalytical Methods

Compound

LLOQ

ULOQ

Species & Matrix

Type

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