In recent years, the revolution in automated bioanalytical approaches has enabled the routine generation of rapid ADME data, providing key information on the metabolism of novel compounds. Amongst the most recent initiatives within the DMPK arena is a drive towards the simultaneous generation of quantitative and qualitative metabolism data, known as ‘qual/quan’ approaches. This work describes an investigation of the benefits and limitations of different mass spectrometric platforms and chromatographic approaches to the generation of qual/quan metabolism data.