Metabolism

Unilabs BIoanalytical Solutions can provide a full metabolite characterisation service from early discovery in vitro experiments to late development radiolabelled studies.

In our new laboratory in Sandwich, UK, we can provide both data and knowledge to clarify key biotransformation issues:

• Metabolic clearance and enzymology
• Sites of metabolic vulnerability
• Bioactivation
• Pharmacologically active metabolites
• Metabolite safety, including MIST

Using state-of-the-art instrumentation, including Thermo LTQ Orbitrap and Waters Q-ToF Premier mass spectrometers, Waters Acquity and Agilent 1290 high-resolution LC systems and Microbeta plate counters, we undertake the following core activities to support discovery and development programs:

• In vitro incubations, including microsomes, cytosol, S9 fractions and hepatocytes in multiple species
• Metabolite identification using LC-HRMS, chemical derivatisation and NMR
• Quantitative analysis of parent compound and metabolites using LC-MS/MS, NMR, UV and radiochemical detection
• Metabolite generation and isolation